University Catalogs

This molecular biology laboratory course is designed to give students hands-on experience performing common techniques used in modern molecular biology, as well as the background information needed to understand what kind of information can be obtained by using them. Because of the dual nature of this course, a portion of the laboratory time will be spent on lectures explaining the theory behind the techniques being used as well as practical aspects of experimental design. In addition, readings will be assigned that explain the history and principles behind some of the techniques used. Basic recombinant DNA techniques: methods for growing, isolating, and purifying recombinant DNA and cloning vectors, DNA sequencing and sequence analysis, gene expression, Polymerase Chain Reaction (PCR), other current techniques. prereq: Biol 3015 or Biol 3020 or Biol 3025 or Bioc 3021 or Bioc 3022 or Bioc 4331 or Biol 4003 or instructor consent